Initial observations on using magnesium metal in peripheral nerve repair.
Identifieur interne : 000460 ( Main/Exploration ); précédent : 000459; suivant : 000461Initial observations on using magnesium metal in peripheral nerve repair.
Auteurs : J J Vennemeyer [États-Unis] ; T. Hopkins [États-Unis] ; M. Hershcovitch [États-Unis] ; K D Little [États-Unis] ; M C Hagen [États-Unis] ; D. Minteer [États-Unis] ; D B Hom [États-Unis] ; K. Marra [États-Unis] ; S K Pixley [États-Unis]Source :
- Journal of biomaterials applications [ 1530-8022 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Hydrogels, Implant résorbable, Kératines, Lésions des nerfs périphériques (), Lésions des nerfs périphériques (anatomopathologie), Lésions des nerfs périphériques (métabolisme), Magnésium, Matériaux biocompatibles, Microtomographie aux rayons X, Muscles squelettiques (anatomopathologie), Muscles squelettiques (innervation), Mâle, Nerf ischiatique (), Nerf ischiatique (métabolisme), Nerf ischiatique (traumatismes), Polyesters, Rats, Rats de lignée LEW, Régénération nerveuse, Test de matériaux.
- MESH :
- anatomopathologie : Lésions des nerfs périphériques, Muscles squelettiques.
- métabolisme : Lésions des nerfs périphériques, Nerf ischiatique.
- traumatismes : Muscles squelettiques, Nerf ischiatique.
- Animaux, Hydrogels, Implant résorbable, Kératines, Lésions des nerfs périphériques, Magnésium, Matériaux biocompatibles, Microtomographie aux rayons X, Mâle, Nerf ischiatique, Polyesters, Rats, Rats de lignée LEW, Régénération nerveuse, Test de matériaux.
English descriptors
- KwdEn :
- Absorbable Implants, Animals, Biocompatible Materials, Hydrogels, Keratins, Magnesium, Male, Materials Testing, Muscle, Skeletal (innervation), Muscle, Skeletal (pathology), Nerve Regeneration, Peripheral Nerve Injuries (metabolism), Peripheral Nerve Injuries (pathology), Peripheral Nerve Injuries (surgery), Polyesters, Rats, Rats, Inbred Lew, Sciatic Nerve (injuries), Sciatic Nerve (metabolism), Sciatic Nerve (surgery), X-Ray Microtomography.
- MESH :
- chemical : Biocompatible Materials, Hydrogels, Keratins, Magnesium, Polyesters.
- injuries : Sciatic Nerve.
- innervation : Muscle, Skeletal.
- metabolism : Peripheral Nerve Injuries, Sciatic Nerve.
- pathology : Muscle, Skeletal, Peripheral Nerve Injuries.
- surgery : Peripheral Nerve Injuries, Sciatic Nerve.
- Absorbable Implants, Animals, Male, Materials Testing, Nerve Regeneration, Rats, Rats, Inbred Lew, X-Ray Microtomography.
Abstract
Biodegradable magnesium metal filaments placed inside biodegradable nerve conduits might provide the physical guidance support needed to improve the rate and extent of regeneration of peripheral nerves across injury gaps. In this study, we examined basic issues of magnesium metal resorption and biocompatibility by repairing sub-critical size gap injuries (6 mm) in one sciatic nerve of 24 adult male Lewis rats. Separated nerve stumps were connected with poly(caprolactone) nerve conduits, with and without magnesium filaments (0.25 mm diameter, 10 mm length), with two different conduit filler substances (saline and keratin hydrogel). At 6 weeks after implantation, magnesium degradation was examined by micro-computed tomography and histological analyses. Magnesium degradation was significantly greater when the conduits were filled with an acidic keratin hydrogel than with saline (p < 0.05). But magnesium filaments in some animals remained intact for 6 weeks. Using histological and immunocytochemical analyses, good biocompatibility of the magnesium implants was observed at 6 weeks, as shown by good development of regenerating nerve mini-fascicles and only mild inflammation in tissues even after complete degradation of the magnesium. Nerve regeneration was not interrupted by complete magnesium degradation. An initial functional evaluation, determination of size recovery of the gastrocnemius muscle, showed a slight improvement due to magnesium with the saline but not the keratin filler, compared with respective control conduits without magnesium. These results suggest that magnesium filament implants have the potential to improve repair of injured peripheral nerve defects in this rodent model.
DOI: 10.1177/0885328214553135
PubMed: 25281648
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000532
- to stream PubMed, to step Curation: 000532
- to stream PubMed, to step Checkpoint: 000532
- to stream Ncbi, to step Merge: 003868
- to stream Ncbi, to step Curation: 003868
- to stream Ncbi, to step Checkpoint: 003868
- to stream Main, to step Merge: 000460
- to stream Main, to step Curation: 000460
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Initial observations on using magnesium metal in peripheral nerve repair.</title><author><name sortKey="Vennemeyer, J J" sort="Vennemeyer, J J" uniqKey="Vennemeyer J" first="J J" last="Vennemeyer">J J Vennemeyer</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biomedical Engineering, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hopkins, T" sort="Hopkins, T" uniqKey="Hopkins T" first="T" last="Hopkins">T. Hopkins</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cellular Physiology, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Physiology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hershcovitch, M" sort="Hershcovitch, M" uniqKey="Hershcovitch M" first="M" last="Hershcovitch">M. Hershcovitch</name><affiliation wicri:level="2"><nlm:affiliation>Department of Otolaryngology-Head & Neck Surgery, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Otolaryngology-Head & Neck Surgery, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Little, K D" sort="Little, K D" uniqKey="Little K" first="K D" last="Little">K D Little</name><affiliation wicri:level="2"><nlm:affiliation>Department of Orthopaedic Surgery, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Orthopaedic Surgery, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hagen, M C" sort="Hagen, M C" uniqKey="Hagen M" first="M C" last="Hagen">M C Hagen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Minteer, D" sort="Minteer, D" uniqKey="Minteer D" first="D" last="Minteer">D. Minteer</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioengineering, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioengineering, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Hom, D B" sort="Hom, D B" uniqKey="Hom D" first="D B" last="Hom">D B Hom</name><affiliation wicri:level="2"><nlm:affiliation>Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology-Head & Neck Surgery and Dermatology, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology-Head & Neck Surgery and Dermatology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Marra, K" sort="Marra, K" uniqKey="Marra K" first="K" last="Marra">K. Marra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioengineering and Department of Plastic Surgery, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioengineering and Department of Plastic Surgery, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Pixley, S K" sort="Pixley, S K" uniqKey="Pixley S" first="S K" last="Pixley">S K Pixley</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cellular Physiology, University of Cincinnati, OH, USA sarah.pixley@uc.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Physiology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25281648</idno><idno type="pmid">25281648</idno><idno type="doi">10.1177/0885328214553135</idno><idno type="wicri:Area/PubMed/Corpus">000532</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000532</idno><idno type="wicri:Area/PubMed/Curation">000532</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000532</idno><idno type="wicri:Area/PubMed/Checkpoint">000532</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000532</idno><idno type="wicri:Area/Ncbi/Merge">003868</idno><idno type="wicri:Area/Ncbi/Curation">003868</idno><idno type="wicri:Area/Ncbi/Checkpoint">003868</idno><idno type="wicri:Area/Main/Merge">000460</idno><idno type="wicri:Area/Main/Curation">000460</idno><idno type="wicri:Area/Main/Exploration">000460</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Initial observations on using magnesium metal in peripheral nerve repair.</title><author><name sortKey="Vennemeyer, J J" sort="Vennemeyer, J J" uniqKey="Vennemeyer J" first="J J" last="Vennemeyer">J J Vennemeyer</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biomedical Engineering, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hopkins, T" sort="Hopkins, T" uniqKey="Hopkins T" first="T" last="Hopkins">T. Hopkins</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cellular Physiology, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Physiology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hershcovitch, M" sort="Hershcovitch, M" uniqKey="Hershcovitch M" first="M" last="Hershcovitch">M. Hershcovitch</name><affiliation wicri:level="2"><nlm:affiliation>Department of Otolaryngology-Head & Neck Surgery, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Otolaryngology-Head & Neck Surgery, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Little, K D" sort="Little, K D" uniqKey="Little K" first="K D" last="Little">K D Little</name><affiliation wicri:level="2"><nlm:affiliation>Department of Orthopaedic Surgery, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Orthopaedic Surgery, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Hagen, M C" sort="Hagen, M C" uniqKey="Hagen M" first="M C" last="Hagen">M C Hagen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology and Laboratory Medicine, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Minteer, D" sort="Minteer, D" uniqKey="Minteer D" first="D" last="Minteer">D. Minteer</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioengineering, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioengineering, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Hom, D B" sort="Hom, D B" uniqKey="Hom D" first="D B" last="Hom">D B Hom</name><affiliation wicri:level="2"><nlm:affiliation>Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology-Head & Neck Surgery and Dermatology, University of Cincinnati, OH, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology-Head & Neck Surgery and Dermatology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Marra, K" sort="Marra, K" uniqKey="Marra K" first="K" last="Marra">K. Marra</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioengineering and Department of Plastic Surgery, University of Pittsburgh, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioengineering and Department of Plastic Surgery, University of Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">Pittsburgh</settlement></placeName><orgName type="university">Université de Pittsburgh</orgName></affiliation></author><author><name sortKey="Pixley, S K" sort="Pixley, S K" uniqKey="Pixley S" first="S K" last="Pixley">S K Pixley</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cellular Physiology, University of Cincinnati, OH, USA sarah.pixley@uc.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Physiology, University of Cincinnati, OH</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author></analytic><series><title level="j">Journal of biomaterials applications</title><idno type="eISSN">1530-8022</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Absorbable Implants</term><term>Animals</term><term>Biocompatible Materials</term><term>Hydrogels</term><term>Keratins</term><term>Magnesium</term><term>Male</term><term>Materials Testing</term><term>Muscle, Skeletal (innervation)</term><term>Muscle, Skeletal (pathology)</term><term>Nerve Regeneration</term><term>Peripheral Nerve Injuries (metabolism)</term><term>Peripheral Nerve Injuries (pathology)</term><term>Peripheral Nerve Injuries (surgery)</term><term>Polyesters</term><term>Rats</term><term>Rats, Inbred Lew</term><term>Sciatic Nerve (injuries)</term><term>Sciatic Nerve (metabolism)</term><term>Sciatic Nerve (surgery)</term><term>X-Ray Microtomography</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Hydrogels</term><term>Implant résorbable</term><term>Kératines</term><term>Lésions des nerfs périphériques ()</term><term>Lésions des nerfs périphériques (anatomopathologie)</term><term>Lésions des nerfs périphériques (métabolisme)</term><term>Magnésium</term><term>Matériaux biocompatibles</term><term>Microtomographie aux rayons X</term><term>Muscles squelettiques (anatomopathologie)</term><term>Muscles squelettiques (innervation)</term><term>Mâle</term><term>Nerf ischiatique ()</term><term>Nerf ischiatique (métabolisme)</term><term>Nerf ischiatique (traumatismes)</term><term>Polyesters</term><term>Rats</term><term>Rats de lignée LEW</term><term>Régénération nerveuse</term><term>Test de matériaux</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biocompatible Materials</term><term>Hydrogels</term><term>Keratins</term><term>Magnesium</term><term>Polyesters</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Lésions des nerfs périphériques</term><term>Muscles squelettiques</term></keywords><keywords scheme="MESH" qualifier="injuries" xml:lang="en"><term>Sciatic Nerve</term></keywords><keywords scheme="MESH" qualifier="innervation" xml:lang="en"><term>Muscle, Skeletal</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Peripheral Nerve Injuries</term><term>Sciatic Nerve</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lésions des nerfs périphériques</term><term>Nerf ischiatique</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Muscle, Skeletal</term><term>Peripheral Nerve Injuries</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Peripheral Nerve Injuries</term><term>Sciatic Nerve</term></keywords><keywords scheme="MESH" qualifier="traumatismes" xml:lang="fr"><term>Muscles squelettiques</term><term>Nerf ischiatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Absorbable Implants</term><term>Animals</term><term>Male</term><term>Materials Testing</term><term>Nerve Regeneration</term><term>Rats</term><term>Rats, Inbred Lew</term><term>X-Ray Microtomography</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Hydrogels</term><term>Implant résorbable</term><term>Kératines</term><term>Lésions des nerfs périphériques</term><term>Magnésium</term><term>Matériaux biocompatibles</term><term>Microtomographie aux rayons X</term><term>Mâle</term><term>Nerf ischiatique</term><term>Polyesters</term><term>Rats</term><term>Rats de lignée LEW</term><term>Régénération nerveuse</term><term>Test de matériaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biodegradable magnesium metal filaments placed inside biodegradable nerve conduits might provide the physical guidance support needed to improve the rate and extent of regeneration of peripheral nerves across injury gaps. In this study, we examined basic issues of magnesium metal resorption and biocompatibility by repairing sub-critical size gap injuries (6 mm) in one sciatic nerve of 24 adult male Lewis rats. Separated nerve stumps were connected with poly(caprolactone) nerve conduits, with and without magnesium filaments (0.25 mm diameter, 10 mm length), with two different conduit filler substances (saline and keratin hydrogel). At 6 weeks after implantation, magnesium degradation was examined by micro-computed tomography and histological analyses. Magnesium degradation was significantly greater when the conduits were filled with an acidic keratin hydrogel than with saline (p < 0.05). But magnesium filaments in some animals remained intact for 6 weeks. Using histological and immunocytochemical analyses, good biocompatibility of the magnesium implants was observed at 6 weeks, as shown by good development of regenerating nerve mini-fascicles and only mild inflammation in tissues even after complete degradation of the magnesium. Nerve regeneration was not interrupted by complete magnesium degradation. An initial functional evaluation, determination of size recovery of the gastrocnemius muscle, showed a slight improvement due to magnesium with the saline but not the keratin filler, compared with respective control conduits without magnesium. These results suggest that magnesium filament implants have the potential to improve repair of injured peripheral nerve defects in this rodent model.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Vennemeyer, J J" sort="Vennemeyer, J J" uniqKey="Vennemeyer J" first="J J" last="Vennemeyer">J J Vennemeyer</name></region><name sortKey="Hagen, M C" sort="Hagen, M C" uniqKey="Hagen M" first="M C" last="Hagen">M C Hagen</name><name sortKey="Hershcovitch, M" sort="Hershcovitch, M" uniqKey="Hershcovitch M" first="M" last="Hershcovitch">M. Hershcovitch</name><name sortKey="Hom, D B" sort="Hom, D B" uniqKey="Hom D" first="D B" last="Hom">D B Hom</name><name sortKey="Hopkins, T" sort="Hopkins, T" uniqKey="Hopkins T" first="T" last="Hopkins">T. Hopkins</name><name sortKey="Little, K D" sort="Little, K D" uniqKey="Little K" first="K D" last="Little">K D Little</name><name sortKey="Marra, K" sort="Marra, K" uniqKey="Marra K" first="K" last="Marra">K. Marra</name><name sortKey="Minteer, D" sort="Minteer, D" uniqKey="Minteer D" first="D" last="Minteer">D. Minteer</name><name sortKey="Pixley, S K" sort="Pixley, S K" uniqKey="Pixley S" first="S K" last="Pixley">S K Pixley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Amérique/explor/PittsburghV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000460 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000460 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Amérique
|area= PittsburghV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25281648
|texte= Initial observations on using magnesium metal in peripheral nerve repair.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25281648" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PittsburghV1
| This area was generated with Dilib version V0.6.38. |  |